A federal jury in Texas sided Thursday with Cisco Systems Inc. (CSCO) in a patent-infringement case brought by VirnetX Holding Corp. (VHC), sending shares of the patent-holding company down 28%.

The verdict from a jury in the U.S. court for Texas' Eastern District dealt a serious blow to VirnetX, a Zephyr Cove, Nev., company that uses its patent portfolio to seek revenue from licensing fees or court judgments. Most of VirnetX's revenue have come from a $200 million settlement reached with Microsoft Corp. (MSFT) in 2010, according to research firm ISI Group.

VirnetX shares fell $9.92 to $25.75 Thursday, erasing more than $500 million of market value. A spokesman for the company didn't immediately respond to a call for comment.

The win for Cisco comes after the Eastern District court last month denied an effort by Apple Inc. (AAPL) to reduce a $368 million award the jury granted for alleged infringement of the same patent.

Thursday's decision didn't directly affect the ruling against Apple, and the court has denied Apple's request for a new trial on the liability and damages portion of the verdict.

The case against Cisco, launched in 2010, accused the networking company of violating one of its patents by selling routers designed to run virtual private networks. Businesses and institutions rely on VPNs to keep their users' Internet activity secure.

VirnetX had prevailed last month when the U.S. Patent and Trademark Office rejected Cisco's challenge of the patent's validity. Cisco Chief Executive John Chambers has criticized some technology companies for filing intellectual property lawsuits that are seen as stifling innovation.

Cisco shares ended little changed at $21.59.

Write to Drew FitzGerald at andrew.fitzgerald@dowjones.com

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