SAN ANTONIO, June 6, 2016 /PRNewswire/ -- At the
64th ASMS Conference, Bruker introduces an entirely new
mass spectrometry technology platform: the innovative
timsTOF™ system combines very high ion mobility
resolution greater than 200, using Bruker's proprietary Trapped
Ion Mobility Spectrometry (TIMS), with Bruker's ultra-high
performance ESI-QTOF mass spectrometry.
The new timsTOF is a flexible, research-grade
instrument for optimal separation and analysis of unresolved
compounds and conformations. Bruker's unique Ion Mobility
Expansion (imeXTM) TIMS technology empowers
market-leading, highest ion mobility resolution that can be
adjusted to research or analytical needs. A novel Open Data
Format supports user-created software tools tailored to specific
research questions.
In recent years, ion mobility separation has matured into a
valuable analytical tool that boosts mass spectrometry analysis in
various areas. Ion mobility resolution, however, has been
restricted in previous IMS systems by physical size limitations.
Bruker's completely unique and proprietary
timsTOF system eliminates these trade-offs and
limitations with the extremely powerful, yet very compact TIMS
device that achieves market-leading ion mobility resolution greater
than 200 for unparalleled separation and IMS research power.
Dr. Melvin Park, Director of
Research at Bruker Daltonics, who has overseen the research of
timsTOF since its inception, has collaborated with key customers to
incorporate further TIMS refinements and develop advanced TIMS
applications. Dr. Park stated: "The all-new timsTOF
system takes ion mobility performance to the next level.
Years of experience in the application of TIMS design and
solid engineering are merged in this novel instrument to set new
standards in ion mobility resolution. Our collaborators and
timsTOF customers will experience unprecedented research
flexibility and exceptional analytical confidence."
Professor Christian Bleiholder in
the Department of Chemistry & Biochemistry at
Florida State University in Tallahassee, FL, added: "Our work focuses on
native structure elucidation of proteins and on studying
protein-ligand binding events by ion mobility spectrometry – mass
spectrometry methods. The high ion mobility resolving
power of our timsTOF system, which routinely exceeds 200, increases
the confidence in the structural interpretation of our data.
Also very important for our work is the ability of TIMS to preserve
solution-phase structures, and the ability to trap analyte ions in
the TIMS device to monitor structural changes over extended periods
of time."
About the timsTOF system
The proprietary timsTOF system offers next-generation of
ion mobility technology to enable the high-definition profiling of
compounds and conformations in complex samples. In addition
to Bruker's high-performance QTOF high mass resolution, ppm
accurate mass and high isotopic fidelity (True Isotopic Pattern, or
TIPTM), TIMS allows separation of isomeric compounds,
accurate determination of collisional cross sections and gas-phase
protein structure and aggregation studies. Furthermore,
fragmentation of TIMS-separated precursor ions results in clear
fragmentation spectra for confident identifications.
Dr. Jan Jordens, at DSM Resolve
in The Netherlands, commented:
"The timsTOF gave us more insight into our samples through
highest resolution LC-TIMS-MS/MS data right from the beginning. We
saw information that we did not even expect. Now, the platform
really helps us to get new knowledge about processes that are
taking place in our samples and also about unexpected
impurities."
Powered by Bruker's Ion Mobility Expansion (imeX) TIMS
technology, the ion mobility resolution of the timsTOF system can
be adjusted to examine compounds of interest in detail. This
powerful TIMS flexibility enables novel workflows which leverage
the unprecedented TIMS resolving power of greater than 200.
Sensitivity has been restricted in previous devices by the
limited duty cycle, which means a significant loss of ions in
commonly used ion mobility devices. The timsTOF system
now enables ion mobility separation with up to 100% duty cycle
based on its unique parallel accumulation capability. TIMS
can be disabled in the timsTOF under software control
without any loss of QTOF performance.
To maximize timsTOF research flexibility, the platform
uses an Open Data Format (*.tdf) that supports tailored analyses
based on open source SQLite, thus enabling users to develop their
own analytical algorithms. With standardized structure and
straightforward access, data from timsTOF are easily
incorporated into innovative data processing, new forms of
visualization and informative reports. The Bruker-supplied Data
Analysis 5.0 software enables interactive analysis of
high-resolution ion mobility data in heat maps, mobilograms, and
mass spectra.
Dr. Carlos Afonso at the
Université de Rouen in France
commented: "We are already using ion mobility in our lab, but with
the much higher ion mobility resolution that timsTOF
provides now, there is a lot of room for different applications
based on this novel technology."
About the Bruker Corporation
For more than 50 years, Bruker has enabled scientists to make
breakthrough discoveries and develop new applications that improve
the quality of human life. Bruker's high-performance
scientific research instruments and high-value analytical solutions
enable scientists to explore life and materials at molecular,
cellular and microscopic levels.
In close cooperation with our customers, Bruker is enabling
innovation, productivity and customer success in life science
molecular research, in applied and pharma applications, in
microscopy, nano-analysis and industrial applications, as well as
in cell biology, preclinical imaging, clinical research,
microbiology and molecular diagnostics. For more information,
please visit: www.bruker.com
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SOURCE Bruker Corporation